Foreword this edition of the difco manual, the eleventh published since 1927, has been extensively revised and rewritten. Brain heart infusion agars brain heart infusion agar. Isolate and detect listeria monocytogenes from clinical, food and environmental samples with the diagnostic thermo scientific remel modified oxford agar mox. Rosebengal in the medium controls the size and height of mold colonies preventing overgrowth and making colony counting. Bd offers a onestop resource for all of your microbiology research needs.
Bacto agar is used in preparing microbiological culture media. Micro agar can be used in a minimal concentration of 5. Dotted lines on maps represent approximate border lines for which there may. Bordetellosis shares many of the clinical and histopathological features of disease caused in mammals by bordetella pertussis and bordetella bronchiseptica. Bacto agar is a solidifying agent in which extraneous matter, pigmented portions and salts have been reduced to a minimum. Pseudomonas f agar is used to detect pigment production by pseudomonas species. The medium relies upon the selective inhibitory properties of sodium azide and the incorporation of tetrazolium that most organisms growing on this medium will reduce to some extent under the conditions provided. Urea agar base, detection of urease producing bacteria.
The purpose of the manual is to provide information about products used in microbiology. Ygc agar yeast extract glucose chloramphenicol agar filidf merck microbiology manual 12th edition 521 this culture medium complies with the recommendations of the international dairy federation internationaler milchwirtschaftsverband 1990, the international organization for standardization iso, the din norm 10186 for the examination. B difco bordetella antisera difco bordetella antigens. Manual of microbiological culture media and a great selection of related books, art and collectibles available now at. Micro agar is a purified agar with a high gel strength and excellent properties for use in plant cell and tissue culture as wel as microbiological work. Quality certificate types offered vary by bd segment, unit, and product line. The two probe system can operate under two strategies. Agar difco mei 100 g from, your source for laboratory equipment and scientific supplies. Improved selective isolation of bordetella pertussis by. Dichloran rosebengal chloramphenicol drbc agar base. Bordet gengou agar base, with the addition of glycerol and. Enumeration of bacteria in water, wastewater, food and dairy. Bd diagnostic 241210 difco mycoplasma agar pplo agar.
Laboratory manual for the diagnosis of whooping cough caused by. Plate count agar difco 247940 online ordering is required to receive these discount low prices. Iso ygc agar yeast extract glucose chloramphenicol agar. When rehydrated and used as described, each vial of bd difco bordetella pertussis and bd difco bordetella parapertussis antisera diluted 1. Pdf bordetella avium virulence measured in vivo and in vitro. Certain formulations are designated by a in the medium name. Manual of microbiological culture media becton dickinson. Bd provides quality certificates for many products certifying that these products have been manufactured and tested in accordance with bd specifications.
Lee and mcclain modified oxford agar by replacing the. With products from the venerated bacto, difco and bbl lines, bd offers peptoneshydrolysates and ingredients manufactured from meat animal tissue, collagen, gelatin, casein and from animalfree materials. A agars bacto agar agar, grade a agar, granulated agar. Agar granulated is qualified to grow recombinant strains of escherichia coli hb101 and saccharomyces cerevisiae. In 1989, curtis described oxford agar, a selective medium for listeria monocytogenes1. Summary and explanation liquid milk is a highly perishable foodstuff with a shelf life of only 510 days after pasteurization.
However, the solubility, gelation temperature and solidity are carefully monitored to permit its use. This agar was developed to address the special needs of the biotechnology industry for large scale. For the cultivation of bacteria and for the enumeration of organisms in water, sewage, feces and other materials. Pseudomonas produce a variety of pigments, and fluorescein is commonly produced by pseudomonas aeruginosa. Bam compf iso smd in vitro diagnosticum for professional. Difco egg yolk enrichment 50% is a concentrated egg yolk emulsion recommended for use in a variety of media such as difco sfp agar base and difco mcclung toabe agar base for the isolation and identification of clostridium species on the basis of their lecithinase activity. Bd difco microbiology difco culture media eugon agar difco 258910 500g. Composition ingredients gms litre proteose peptone 5. Bordet gengou blood agar contains potato infusion and glycerol to supply the nutrients necessary to support the growth of b. The bacteria were harvested in magnesiumfree earles balanced salt solution ebss sigma chemical co. Dichloran rosebengal chloramphenicol drbc iso is a dehydrated culture medium formulated according to bs iso 215271. Diagnostic tests and clinical products microbiology microbiology media, stains and supplies dehydrated media. Manual tank gauging is performed using the portable agar id310.
Noble agar is perfect for electrophoretic and nutritional procedures wherever increased purity is required. Bd difco agar, technical is a solidifying agent with wider quality control parameters than other bacteriological agars. Special installation may require the use of the agar id202 for extended length. Bacto bordet gengou agar base is used with added blood for isolating. Border gengou agar description a selective media for the isolation of bordatella pertussis and bordatella parapertussis from clinical specimens.
Atcc 8467 identity specifications difco bordet gengou agar base dehydrated. This chapter describes menterococcus me agar as an improved version of the original medium of slanetz. If growth is observed on pseudomonas f agar, fluorescein production is determined by visual examination of the. Potato dextrose agar difco dehydrated culture media and ingredients. User quality control identity specifications difco myp agar dehydrated appearance. Resistance to bordetella bronchiseptica infection in swine. Difco laboratories, division of becton dickinson and company sparks, maryland 21152 usa vi the difco manual foreword. Difco bbl manual manual of microbiological culture media. Potato dextrose agar difco dehydrated culture media and. Bd bactec plus anaerobicf medium culture vials prereduced. Bordetella avium causes an upperrespiratorytract disease called bordetellosis in birds.
Ss agar salmonella shigella agar m108d ss agar salmonella shigella agar is a differential selective media used for the isolation of salmonella and some shigella species from pathological specimens, suspected foodstuffs etc. Bd supplies greater than 400 different difco and bbl brand media formulations in dehydrated form for the convenience of the laboratorian and bioprocessing specialist. Bordet gengou agar base, with the addition of glycerol and sterile blood, is used. In this study we determined several parameters of infection in the domestic turkey, meleagris galapavo, and compared these in vivo findings with an in. Defibrinated animal blood supplies additional nutrients and enables the detection of hemolytic reactions, which aid in the identification of b. Bordet gengou agar bg agar was the standard culture medium for isolation of b.
There are no alternate images available for this product. Bd difco yeast mold agar is prepared according to the formulae published by wickerham. The isolates were cultured on bordet gengou agar difco or cyclodextrin solid medium csm agar 31, and incubated at 36uc for 223 days. Out of 290 yeast colonies isolated on sabouraud agar, corn meal agar stimulated the production of chlamydospores in 149 colonies 51%, soil extract agar in 103 36% and purified polysaccharide medium in 94 32%. Bordetella avium virulence measured in vivo and in vitro ncbi nih. Bordetella avium virulence measured in vivo and in vitro. Detrimental ions such as iron and copper are reduced. Bacto agar difco media distributor pharmaceuticals. Bordet gengou blood agar is used in clinical laboratories as a method of diagnosing whooping cough.
Intended use hardy diagnostics pc pseudomonas cepacia agar is recommended for the selective isolation of burkholderia pseudomonas cepacia from clinical and environmental materials. Pdf comparison of bloodfree medium cyclodextrin solid. Bacto agar is optimized for beneficial calcium and magnesium content. Bordetella pertussis, the etiologic agent of this disease, may be isolated from user quality control identity specifications difco bordet gengou agar base dehydrated appearance. Gengou agar containing nalidixic acid 30 m gml and 15% sheep blood at 35c. Solidifying agent essentially free of impurities used for electrophoretic and nutritional procedures wherever increased purity is required. Bordetgengou agar was prepared as directed difco with 15% sheep blood. Tracheae from the shaminoculated birds had a welldefined ciliated border with.
Dichloran rosebengal chloramphenicol drbc agar base is based on the formulation described by king et al. Agar granulated may be used for general bacteriological purposes where clarity is not a strict requirement. Usually an agar id201 probe is inserted into the tank through agars patented seal housing, as shown in figure 1. Bacto agar agar, grade a agar, granulated agar, technical agar, noble agarose agar, select intended use bacto agar is a solidifying agent in which extraneous matter, pigmented portions and salts have been reduced to a minimum. Difco bairdparker agar base aoac bam ccam compf iso smd smww usda cat. Bd is a leading medical technology company that advances human health in every corner of the world by improving medical research, diagnostics and the delivery of care. Suspend 46 g of the powder in 900 ml of purified water. Peptic digest of animal tissue, proteose peptone and yeast extract provide carbon, nitrogen, sulphur, vitamin b complex and other essential growth nutrients. Plate count agar difco 247910 online ordering is required to receive these discount low prices.
For the chromogenic detection and enumeration of enterococci in water by the membrane filter technique. If growth is observed on pseudomonas f agar, fluorescein. Bordet gengou medium by loss of haemolysis and different aspect of colonies. Agar, noble difco dehydrated culture media and ingredients.
1422 479 486 252 834 471 1220 435 184 1537 959 1228 200 1221 173 30 471 574 864 1547 883 712 1420 286 732 1175 1031 1018 366 247 50